I have been researching cancer for over 44 years and I have yet to find anything as safe and effective as ozone therapy for cancer. when used properly. Ozone selectively kills cancer cells through a peroxide overload in a chain reaction, destroys cancer pathogens (viruses, bacteria, etc), destroys many carcinogens through oxidation and increases anticancer cytokines and superoxide dismutase. Ozone also increases the antioxidant enzymes catalase and peroxidases in healthy cells, which is why it will not harm healthy cells when used properly. A study did in Germany followed almost 6 million doses of ozone given in therapy and out of that nearly 6 million there were only just over 30 adverse reactions. Most of these mild such as pain at the injection site or mild rash.
Another advantage of ozone therapy is that it readily enters in to all parts of the body including the brain and cerebral spinal fluid where most drugs and other compounds cannot reach since few things cross the blood brain barrier.
Ozone therapy can be easily done at home by the person themself. With brain tumors though, it is important to go slow as the dead cancer cells form uric acid that irritates the tissues and leads to swelling. Not a god thing if the brain swells in the skull as this can crush the brain. Therefore, when doing ozone for brain tumors, which can be non-invasively through ear insufflation, the treatment is done much slower than for cancers elsewhere in the body.
Hate to say it, but study after study has proven that radiation and chemotherapy rarely ever succeed in completely eliminating cancer. The main reason is that radiation therapy and most chemo drugs, which ironically are carcinogens (cancer causing agents) work on a free radical principle. More specifically an oxygen radical to kill cancer cells. In order to do this, there must first be sufficient oxygen present. A malignant tumors grow though, they form a very irregular vasculature, which leaves some parts of the tumor well oxygenated, but other areas hypoxic. The radiation and most chemotherapy drugs therefore work well in the well oxygenated areas, but do nothing in the hypoxic regions. So, these hypoxic regions survive and will grow back creating more resistant hypoxic regions in the process. This is how radiation and chemo resistant tumors form. Again, this is extremely well documented in the medical journals. Therefore, the radiation and most chemotherapies simply knock back the tumors to undetectable levels, which is known as remission, not cured, as the tumors almost always grow back.
The second issue is that radiation therapy and most chemotherapy drugs do not address the cause of the cancer, which in about 95% of cancers are cancer viruses. To a lesser extent bacteria, mycotoxins, radiation and in extremely rare cases parasites. No cancer has ever been proven to be hereditary and in fact, every oncogene every discovered has been viral. No human oncogene has ever been discovered.
People, including doctors and researchers, often confuse genetic with hereditary. These ARE NOT the same thing. All cancers are genetic as they involve alterations in the genes. To be hereditary though, the person must have inherited the defective gene from one or both parents, which means they have the defective gene and thus the disease/disorder from birth. Not 40, 50, 60..... years down the line. Again, no human oncogene has ever been discovered. The medical establishment still tries to confuse and mislead people by saying ridiculously stupid things such as a person tested positive for the BRCA gene, which has led to many prophylactic mastectomies and prostectomies of non-cancerous tissue. EVERYONE has BRCA genes, these are TUMOR SUPPRESSOR genes, not oncogenes (genes that cause cancer). The BRCA1 and BRCA2 gene mutations are viral mutations where the virus has inserted its own genetic code in to our genes altering the way these genes function. In this case, the viral insertion in to the BRCA genes turns off their tumor suppressor functions.
Brain tumors are also almost always viral in origin. The viruses known to cause brain tumors in humans are simian vacuole virus type 40 (SV40), BK virus, cytomegalovirus (human herpes virus type 5) and the JC virus. To a much lesser extent, brain tumors can occur from bacteria known as mycoplasmas (cell wall deficient bacteria), and is super rare cases the parasite Toxoplasma gondii.
The only cancer therapies with a relatively high success rate, around 80% long term remission to cure, are the drugs derived from herbs or based on their chemistry. Vincristine and Vinblastine from the herb Madagascar periwinkle, and the podophyllumtoxin derivatives (PDs). The PDs were originally derived from the herb mandrake, then from the Savin juniper herb. It was reported in the Journal of the American Medical Association an the Journal of the American Cancer Society back in the early 1950s to be HIGHLY EFFECTIVE for 6 forms of cancer it was tested on, including breast cancer. The compound was not patentable though, and so it went nowhere for over 50 years while people were dying from these curable cancers. Finally, the University of North Carolina synthesized the PDs over 50 years later and sold the patent to a California drug company who finally got approval on the drug over another decade later. The reason Vincristine, Vinblastine and the PDs have such a high efficacy rate for cancer is that these herbal based compounds are powerful antiviral agents and thus actually address the cause of most cancers.
Really quick, the SV40 virus as mentioned is a cause of brain cancers, and has been a major cause of brain cancers, bone cancers and mesothelioma. Multiple studies have found that mesothelioma is not caused from asbestos, but rather SV40, where the asbestos is simply a co-factor, such as an activator like estrogen and progesterone are for many cancer viruses. SV40 is actually a primate virus, so this confuses people as to how humans are getting this cancer causing primate virus. The virus was introduced in to humans in the 50s and 60s from the polio vaccines we were given as children. The vaccines were cultured on green monkey kidneys, which led to contamination of the vaccines with SV40 that over 90 million Americans alone were given. The virus is a lentvirus (slow virus) and thus takes many decades to show symptom such as cancer. This is why there was such as large surge in normally rare cancers such as brain cancers decades after the polio vaccines had been discontinued.
Back to why radiation therapy and most chemotherapy drugs tend to fail. So, part of the answer is lack of efficacy in hypoxic regions, being themselves carcinogenic and failure to address cancer pathogens.
Another factor is metastasized cells. Cancer cells can naturally metastasize, or they can metastasize from procedures such as biopsies or surgeries where the cancer cells can be knocked loose and float through the vascular or lymphatic system to distant parts of the body where they take up residence. There are two very important things to keep in mind here. First is that cancer cells are very efficient in evading immune detection. They have several mechanisms for doing this including using human chorionic gonadotropin as a shield to hide from the immune system just like a human fetus. In fact, cancer cells share a lot of characteristics with human fetuses and can often be picked up by a pregnancy test. Second thing to keep in mind is that malignant tumors also follow the rule of survival of the fittest. The tumor needs a food supply (glucose, fructose, ketones, lactate, fatty acids and some amino acids), as well as oxygen to survive and thrive. The more oxygen available, the faster the cancer can grow. When the tumor first starts to develop it has no vasculature. Therefore, it relies on diffusion to get its oxygen. This is a very limited process though, and once the tumor reaches a size of 2-3mm the tumor can no longer receive sufficient oxygen through diffusion, and the center of the tumor dies from a lack of oxygen. This in turn stimulates the production of vascular growth factors such as vascular endothelial growth factor (VEGF) that stimulates angiogenesis (blood vessel formation) to increase fuel and especially oxygen levels to the tumor allowing it grow more effectively. Same reason malignant tumors in low blood supply and thus oxygen areas, such as the breast, tend to grow extremely slow a where highly oxygenated areas such as the liver and pancreas form very rapidly growing malignant tumors. This brings us to the survival of the fittest part. The primary tumor is not only producing angiogenesis promoters, such as VEGF, but the tumor also at the same time produces small amounts of angiogenesis inhibitors (AIs). This may sound counter intuitive at first, nut there is a reason for this. The AIs float through the bloodstream reaching the metastasized cancer cells slowing or stopping their growth so there is a greater fuel and oxygen supply going to the primary tumor. When the primary tumor is removed though, the source of AIs is also now gone and the metastasized cells are now free to grow unimpeded.
The medical establishment though is deceptive and often counts this, or any original tumor that grows back after the 5 year term considered a "cure" as a new tumor as to make the original therapy appear as a success even though they are the cancer cells. This is one of the ways they manipulate the cancer therapy statistics to make the therapies appear way more effective than they actually are.
Some ozone therapy references:
www.ncbi.nlm.nih.gov/pubmed/22470237Ozone therapy: A clinical review.
Elvis AM1, Ekta JS.
Author information
Abstract
Ozone (O(3)) gas discovered in the mid-nineteenth century is a molecule consisting of three atoms of oxygen in a dynamically unstable structure due to the presence of mesomeric states. Although O(3) has dangerous effects, yet researchers believe it has many therapeutic effects. Ozone therapy has been utilized and heavily studied for more than a century. Its effects are proven, consistent, safe and with minimal and preventable side effects. Medical O(3) is used to disinfect and treat disease. Mechanism of actions is by inactivation of bacteria, viruses, fungi, yeast and protozoa, stimulation of oxygen metabolism, activation of the immune system. Medication forms in a gaseous state are somewhat unusual, and it is for this reason that special application techniques have had to be developed for the safe use of O(3). In local applications as in the treatment of external wounds, its application in the form of a transcutaneous O(3) gas bath has established itself as being the most practical and useful method, for example at low (sub-atmospheric) pressure in a closed system guaranteeing no escape of O(3) into the surrounding air. Ozonized water, whose use is particularly known in dental medicine, is optimally applied as a spray or compress. Diseases treated are infected wounds, circulatory disorders, geriatric conditions, macular degeneration, viral diseases, rheumatism/arthritis, cancer, SARS and AIDS.
Free PMC Article
Int J Cancer. 2008 May 15;122(10):2360-7. doi: 10.1002/ijc.23382.
Treatment with ozone/oxygen-pneumoperitoneum results in complete remission of rabbit squamous cell carcinomas.
Schulz S 1, Häussler U, Mandic R, Heverhagen JT, Neubauer A, Dünne AA, Werner JA, Weihe E, Bette M.
Author information
Abstract
Head and neck squamous cell carcinomas (HNSCC) represent a group of metastasizing tumors with a high mortality rate in man and animals. Since the biomolecule ozone was found to inhibit growth of various carcinoma cells in vitro we here applied the highly aggressive and lethal VX2 carcinoma HNSCC tumor model of the New Zealand White rabbit to test whether ozone exerts antitumorous effects in vivo. Therapeutic insufflation of medical ozone/oxygen (O(3)/O(2)) gas mixture into the peritoneum (O(3)/O(2)-pneumoperitoneum) at an advanced stage of tumor disease led to a survival rate of 7/14 rabbits. Six of the seven surviving rabbits presented full tumor regression and the absence of local or distant lung metastases. Insufflation of pure oxygen (O(2)) resulted in a survival rate of 3/13 animals accompanied by full tumor remission in 2 of the 3 surviving animals. Of the 14 sham-treated animals only 1 had spontaneous tumor remission and survived. No adverse effects or changes in standard blood parameters were observed after repeated intraperitoneal insufflations of the O(3)/O(2) or O(2) gas. Animals with O(3)/O(2)-induced tumor eradication developed tolerance against reimplantation of the VX2 tumor. This could be reversed by immune suppression with a combination of dexamethasone and cyclosporin A suggesting an antitumorous effect of O(3)/O(2)-mediated activation of the body’s own immunosurveillance. Although the exact mechanisms of action are still unclear the present data point to O(3)/O(2)-pneumoperitoneum as a promising new strategy in anticancer therapy.
Evidence-Based Complementary and Alternative Medicine
Volume 1 (2004), Issue 3, Pages 321-325
dx.doi.org/10.1093/ecam/neh038Original Article
Adjuvant Ozonetherapy in Advanced Head and Neck Tumors: A Comparative Study
Bernardino Clavo,1,7 Ana Ruiz,1,7 Marta Lloret,1,7 Laura López,1,7 Gerardo Suárez,1,7 David Macías,2,7 Victor Rodríguez,6 Maria A. Hernández,1,7 Roberto Martín-Oliva,2 Santiago Quintero,3 José M. Cuyás,4 and Francisco Robaina5,7
1Department of Radiation Oncology-Research Unit, Las Palmas, Canary Islands, Spain
2Department of Medical Physics, Las Palmas, Canary Islands, Spain
3Department of Oral and Maxillofacial Surgery, Las Palmas, Canary Islands, Spain
4Department of Otolaryngology, Las Palmas, Canary Islands, Spain
5Department of Neurosurgery and Chronic Pain Unit of the Dr Negrín Hospital, Las Palmas, Canary Islands, Spain
6La Paterna Medical Center Las Palmas, Canary Islands, Spain
7Canary Islands Institute for Cancer Research (ICIC), Las Palmas, Canary Islands, Spain
Received 13 March 2004; Accepted 20 August 2004
Copyright © 2004 Bernardino Clavo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Advanced head and neck (H&N) tumors have a poor prognosis, and this is worsened by the occurrence of hypoxia and ischemia in the tumors. Ozonetherapy has proved useful in the treatment of ischemic syndromes, and several studies have described a potential increase of oxygenation in tissues and tumors. The aim of this prospective study was to evaluate the clinical effect of ozonetherapy in patients with advanced H&N cancer in the course of their scheduled radiotherapy. Over a period of 3 years, 19 patients with advanced H&N tumors who were undergoing treatment in our department with non-standard fractionated radiotherapy plus oral tegafur. A group of 12 patients was additionally treated with intravenous chemotherapy before and/or during radiotherapy. In the other group of seven patients, systemic ozonetherapy was administered twice weekly during radiotherapy. The ozonetherapy group was older (64 versus 54 years old, P = 0.006), with a higher percentage of lymph node involvement (71% versus 8%, P = 0.019) and with a trend to more unfavorable tumor stage (57% versus 8% IVb + IVc stages, P = 0.073). However, there was no significant difference in overall survival between the chemotherapy (median 6 months) and ozonetherapy (8 months) groups. Although these results have to be viewed with caution because of the limited number of patients, they suggest that ozonetherapy could have had some positive effect during the treatment of our patients with advanced H&N tumors. The adjuvant administration of ozonetherapy during the chemo–radiotherapy for these tumors merits further research.
Full article and citing articles available-
www.hindawi.com/journals/ecam/2004/581750/abs/Evidence-Based Complementary and Alternative Medicine
Volume 1 (2004), Issue 1, Pages 93-98
dx.doi.org/10.1093/ecam/neh009Original Article
Ozone Therapy for Tumor Oxygenation: a Pilot Study
Bernardino Clavo,1,5 Juan L. Pérez,2,5 Laura López,1,5 Gerardo Suárez,1,5 Marta Lloret,1,5 Victor Rodríguez,3 David Macías,2,5 Maite Santana,1,5 María A. Hernández,1 Roberto Martín-Oliva,2 and Francisco Robaina4,5
1Radiation Oncology and Research Unit, Las Palmas (Canary Islands), Spain
2Medical Physics, Las Palmas (Canary Islands), Spain
3La Paterna Medical Center, Las Palmas (Canary Islands), Spain
4Chronic Pain Unit, Dr Negrín Hospital, Las Palmas (Canary Islands), Spain
5Canary Islands Institute for Cancer Research (ICIC), Las Palmas (Canary Islands), Spain
Received 17 November 2003; Accepted 4 February 2004
Copyright © 2004 Bernardino Clavo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Tumor hypoxia is an adverse factor for chemotherapy and radiotherapy. Ozone therapy is a non-conventional form of medicine that has been used successfully in the treatment of ischemic disorders. This prospective study was designed to assess the effect of ozone therapy on tumor oxygenation. Eighteen subjects were recruited for the study. Systemic ozone therapy was administered by autohemotransfusion on three alternate days over one week. Tumor oxygenation levels were measured using polarographic needle probes before and after the first and the third ozone therapy session. Overall, no statistically significant change was observed in the tumor oxygenation in the 18 patients. However, a significant decrease was observed in hypoxic values ≤10 and ≤5 mmHg of pO2. When individually assessed, a significant and inverse non-linear correlation was observed between increase in oxygenation and the initial tumor pO2 values at each measuring time-point, thus indicating that the more poorly-oxygenated tumors benefited most (rho = −0.725; P = 0.001). Additionally, the effect of ozone therapy was found to be lower in patients with higher hemoglobin concentrations (rho = −0.531; P < 0.034). Despite being administered over a very short period, ozone therapy improved oxygenation in the most hypoxic tumors. Ozone therapy as adjuvant in chemo-radiotherapy warrants further research.
Full article and citing articles available-
www.hindawi.com/journals/ecam/2004/437019/citations/Mediators Inflamm. 1998;7(5):313-7.
Studies on the biological effects of ozone: 8. Effects on the total antioxidant status and on interleukin-8 production.
Bocci V 1, Valacchi G, Corradeschi F, Fanetti G.
Author information
Abstract
Ozone (O3) is a controversial gas because, owing to its potent oxidant properties, it exerts damaging effects on the respiratory tract and yet it has been used for four decades as a therapy. While the disinfectant activity of O3 is understandable, it is less clear how other biological effects can be elicited in human blood with practically no toxicity. On the other hand plasma and cells are endowed with a powerful antioxidant system so that a fairly wide range of O3 concentrations between 40 and 80 microg/ml per gram of blood (approximately 0.83-1.66 mM) are effective but not deleterious. After blood ozonation total antioxidant status (TAS) and plasma protein thiol groups (PTG) decrease by 20% and 25%, respectively, while thiobarbituric acid reactive substances (TBARS) increases up to five-fold. The increase of haemolysis is negligible suggesting that the erythrocyte membrane is spared at the expense of other sacrificial substrates. While there is a clear relationship between the ozone dose and IL-8 levels, we have noticed that high TAS and PTG values inhibit the cytokine production. This is in line with the current idea that hydrogen peroxide, as a byproduct of O3 decomposition, acts as a messenger for the cytokine induction.
Free PMC Article
J Biol Regul Homeost Agents. 1998 Jul-Sep;12(3):67-75.
Studies on the biological effects of ozone: 7. Generation of reactive oxygen species (ROS) after exposure of human blood to ozone.
Bocci V1, Valacchi G, Corradeschi F, Aldinucci C, Silvestri S, Paccagnini E, Gerli R.
Author information
Abstract
The acceptance of any complementary medical approach is conditioned by the results obtained after the same scientific scrutiny applied in orthodox medicine. Otherwise any claim of efficacy remains in the realm of fiction. In the case of ozone therapy, the mechanisms of action have remained nebulous and in a series of publications we are trying to present the biochemical, immunological and morphological evidence in favour or against ozone therapy. We have now shown that ozone (O3) dissolved in the water of either plasma or serum or physiological saline generates reactive oxygen species (ROS), of which hydrogen peroxide (H2O2) can be unequivocally demonstrated by using specific methods for its detection. Lipids present in plasma preferentially those present in lipoproteins, undergo peroxidation that is somewhat O3-dose dependent and can be observed by the measurement of thiobarbituric acid reactive substances (TBARS). While the generation of H2O2 is crucial in activating both biochemical (hexose monophosphate shunt) and immunological (via the transcription factor NF-kB) mechanisms, the role of lipid oxidation products (LOP) remains to be investigated. We have shown here that there is a small but consistent induction of some cytokines (TNF-alpha, IFN-gamma and IL-2) when human blood is directly exposed to O3 concentrations up to 100 micrograms/ml per g of blood. On the other hand, isolated blood mononuclear cells (PBMC) in tissue culture medium are far more sensitive to the oxidant action of O3 as shown by a progressive reduction of the proliferation index with comparatively far lower O3, concentrations. On the whole, these results support the concept that much of the O3 toxicity is neutralized by the powerful antioxidant system of blood. The minimal hemolysis supports this idea but as far as platelets are concerned, we must mention that they tend to aggregate in heparinized blood, even when it is exposed to an O3 concentration of 40 micrograms/ml. In spite of the lack of side-effects after autohemotherapy, this drawback must be kept in mind and avoided in clinical practice.
Oxygen-ozone therapy in medicine: an update
www.ncbi.nlm.nih.gov/pubmed/19752…00m,isrctn
www.ncbi.nlm.nih.gov/pubmed/22470237Ozone therapy: A clinical review.
Elvis AM1, Ekta JS.
Author information
Abstract
Ozone (O(3)) gas discovered in the mid-nineteenth century is a molecule consisting of three atoms of oxygen in a dynamically unstable structure due to the presence of mesomeric states. Although O(3) has dangerous effects, yet researchers believe it has many therapeutic effects. Ozone therapy has been utilized and heavily studied for more than a century. Its effects are proven, consistent, safe and with minimal and preventable side effects. Medical O(3) is used to disinfect and treat disease. Mechanism of actions is by inactivation of bacteria, viruses, fungi, yeast and protozoa, stimulation of oxygen metabolism, activation of the immune system. Medication forms in a gaseous state are somewhat unusual, and it is for this reason that special application techniques have had to be developed for the safe use of O(3). In local applications as in the treatment of external wounds, its application in the form of a transcutaneous O(3) gas bath has established itself as being the most practical and useful method, for example at low (sub-atmospheric) pressure in a closed system guaranteeing no escape of O(3) into the surrounding air. Ozonized water, whose use is particularly known in dental medicine, is optimally applied as a spray or compress. Diseases treated are infected wounds, circulatory disorders, geriatric conditions, macular degeneration, viral diseases, rheumatism/arthritis, cancer, SARS and AIDS.
Free PMC Article
Does ozone therapy normalize the cellular redox balance? Implications for therapy of human immunodeficiency virus infection and several other diseases.
www.ncbi.nlm.nih.gov/pubmed/8692040Mediators Inflamm. 2004 Dec;13(5-6):307-12.
Reversion by ozone treatment of acute nephrotoxicity induced by cisplatin in rats.
González R1, Borrego A, Zamora Z, Romay C, Hernández F, Menéndez S, Montero T, Rojas E.
Author information
Abstract
BACKGROUND:
Ozone therapy has become a useful treatment for pathological processes, in which the damage mediated by reactive oxygen species is involved. Several lines of evidence suggest that cisplatin-induced acute nephrotoxicity is partially mediated by reactive oxygen species
AIMS:
To analyze the effect of ozone administration after cisplatin-induced acute nephrotoxicity.
METHODS:
Male Sprague-Dawley rats were treated with five intra-rectal applications of ozone/oxygen mixture at 0.36, 1.1 and 1.8 mg/kg after cisplatin intraperitoneal injection (6 mg/kg). Serum and kidneys were taken off 5 days after cisplatin treatment. Creatinine was measured in the serum and the activities of antioxidant enzymes and thiobarbituric acid reactive substances and glutathione content were analyzed in renal homogenate.
RESULTS:
Ozone treatment diminished the increase in serum creatinine levels, the glutathione depletion and also reversed the inhibition of superoxide dismutase, catalase and glutathione peroxidase activities induced by cisplatin in the rat kidney. Also, the renal content of thiobarbituric reactive substances was decreased by ozone/oxygen mixture applied after cisplatin.
CONCLUSION:
Intrarectal applications of ozone reversed the renal pro-oxidant unbalance induced by cisplatin treatment by the way of stimulation to some constituents of antioxidant system in the kidney, and thereby it decreased the renal damage.
Free PMC Article
Mediators Inflamm. 2004 Feb;13(1):13-9.
Protection by ozone preconditioning is mediated by the antioxidant system in cisplatin-induced nephrotoxicity in rats.
Borrego A1, Zamora ZB, González R, Romay C, Menéndez S, Hernández F, Montero T, Rojas E.
Author information
Abstract
BACKGROUND:
Acute renal failure is a dose-limiting factor of cisplatin chemotherapy. Here, we show the protective effect of ozone oxidative preconditioning against cisplatin-induced renal dysfunction in rats. Ozone oxidative preconditioning is a prophylactic approach, which favors the antioxidant-pro-oxidant balance for preservation of the cell redox state by increasing antioxidant endogenous systems in various in vivo and in vitro experimental models.
AIMS:
To analyze the protective role of ozone oxidative preconditioning against cisplatin-induced nephrotoxicity.
METHODS:
Male Sprague-Dawley rats were pretreated with 15 intrarectal applications of ozone/oxygen mixture at 0.36, 0.72, 1.1, 1.8 and 2.5 mg/kg before cisplatin intraperitoneal injection (6 mg/kg). Serum and kidneys were extracted and analyzed 5 days after cisplatin treatment for determinations of the renal content of glutathione, thiobarbituric acid-reactive substances, renal concentration and enzymatic activities of catalase, superoxide dismutase and glutathione peroxidase.
RESULTS:
Ozone pretreatment prevented the increase in serum creatinine levels, the glutathione depletion and the inhibition of superoxide dismutase, catalase and glutathione peroxidase activities induced by cisplatin in the rat kidney. Also, the renal content of thiobarbituric acid-reactive substances was decreased by ozone therapy. These protective effects of ozone were dose dependent.
CONCLUSIONS:
Intrarectal ozone therapy prevented effectively the renal antioxidant unbalance induced by cisplatin treatment.
Free PMC Article
Sultan Qaboos Univ Med J. 2014 Aug;14(3):e342-8. Epub 2014 Jul 24.
Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats.
Delgado-Roche L1, Hernández-Matos Y1, Medina EA1, Morejón DÁ1, González MR2, Martínez-Sánchez G3.
Author information
Abstract
OBJECTIVES:
Induced dilated cardiomyopathy is the main limitation of the anti-cancer drug doxorubicin, which causes oxidative stress and cardiomyocyte death. As ozone therapy can activate the antioxidant systems, this study aimed to investigate the therapeutic efficacy of ozone-oxidative preconditioning against doxorubicin-induced cardiotoxicity.
METHODS:
The study was carried out from September 2013 to January 2014. Sprague-Dawley rats were randomly distributed in the following treatment groups: Group 1 were treated with 2 mg/kg intraperitoneal (i.p.) of doxorubicin twice a week for 50 days; Group 2 were treated with 0.3 mg of ozone/oxygen mixture at 50 μg/mL of ozone per 6 mL of oxygen by rectal insufflation and then treated with doxorubicin; Group 3 were treated as Group 2 but only with the oxygen, and Group 4 were treated with oxygen first, and then with sodium chloride i.p. as the control group.
RESULTS:
The results showed that ozone therapy preserved left ventricle morphology which was accompanied by a reduction of serum pro-brain natriuretic peptide levels. The cardioprotective effects of ozone-oxidative preconditioning were associated with a significant increase (P <0.05) of antioxidant enzymes activities and a reduction of lipid and protein oxidation (P <0.05).
CONCLUSION:
Ozone-oxidative preconditioning prevents doxorubicin-induced dilated cardiomyopathy through an increase of antioxidant enzymes and a reduction of oxidised macromolecules. This establishes the background for future studies to determine if ozone therapy can be used as a complementary treatment for attenuating doxorubicin-induced cardiotoxicity in cancer patients.
Free PMC Article
www.ncbi.nlm.nih.gov/pubmed/22470237Ozone therapy: A clinical review.
Elvis AM1, Ekta JS.
Author information
Abstract
Ozone (O(3)) gas discovered in the mid-nineteenth century is a molecule consisting of three atoms of oxygen in a dynamically unstable structure due to the presence of mesomeric states. Although O(3) has dangerous effects, yet researchers believe it has many therapeutic effects. Ozone therapy has been utilized and heavily studied for more than a century. Its effects are proven, consistent, safe and with minimal and preventable side effects. Medical O(3) is used to disinfect and treat disease. Mechanism of actions is by inactivation of bacteria, viruses, fungi, yeast and protozoa, stimulation of oxygen metabolism, activation of the immune system. Medication forms in a gaseous state are somewhat unusual, and it is for this reason that special application techniques have had to be developed for the safe use of O(3). In local applications as in the treatment of external wounds, its application in the form of a transcutaneous O(3) gas bath has established itself as being the most practical and useful method, for example at low (sub-atmospheric) pressure in a closed system guaranteeing no escape of O(3) into the surrounding air. Ozonized water, whose use is particularly known in dental medicine, is optimally applied as a spray or compress. Diseases treated are infected wounds, circulatory disorders, geriatric conditions, macular degeneration, viral diseases, rheumatism/arthritis, cancer, SARS and AIDS.
Free PMC Article
Int J Cancer. 2008 May 15;122(10):2360-7. doi: 10.1002/ijc.23382.
Treatment with ozone/oxygen-pneumoperitoneum results in complete remission of rabbit squamous cell carcinomas.
Schulz S1, Häussler U, Mandic R, Heverhagen JT, Neubauer A, Dünne AA, Werner JA, Weihe E, Bette M.
Author information
Abstract
Head and neck squamous cell carcinomas (HNSCC) represent a group of metastasizing tumors with a high mortality rate in man and animals. Since the biomolecule ozone was found to inhibit growth of various carcinoma cells in vitro we here applied the highly aggressive and lethal VX2 carcinoma HNSCC tumor model of the New Zealand White rabbit to test whether ozone exerts antitumorous effects in vivo. Therapeutic insufflation of medical ozone/oxygen (O(3)/O(2)) gas mixture into the peritoneum (O(3)/O(2)-pneumoperitoneum) at an advanced stage of tumor disease led to a survival rate of 7/14 rabbits. Six of the seven surviving rabbits presented full tumor regression and the absence of local or distant lung metastases. Insufflation of pure oxygen (O(2)) resulted in a survival rate of 3/13 animals accompanied by full tumor remission in 2 of the 3 surviving animals. Of the 14 sham-treated animals only 1 had spontaneous tumor remission and survived. No adverse effects or changes in standard blood parameters were observed after repeated intraperitoneal insufflations of the O(3)/O(2) or O(2) gas. Animals with O(3)/O(2)-induced tumor eradication developed tolerance against reimplantation of the VX2 tumor. This could be reversed by immune suppression with a combination of dexamethasone and cyclosporin A suggesting an antitumorous effect of O(3)/O(2)-mediated activation of the body's own immunosurveillance. Although the exact mechanisms of action are still unclear the present data point to O(3)/O(2)-pneumoperitoneum as a promising new strategy in anticancer therapy.
Evidence-Based Complementary and Alternative Medicine
Volume 1 (2004), Issue 3, Pages 321-325
dx.doi.org/10.1093/ecam/neh038 Original Article
Adjuvant Ozonetherapy in Advanced Head and Neck Tumors: A Comparative Study
Bernardino Clavo,1,7 Ana Ruiz,1,7 Marta Lloret,1,7 Laura López,1,7 Gerardo Suárez,1,7 David Macías,2,7 Victor Rodríguez,6 Maria A. Hernández,1,7 Roberto Martín-Oliva,2 Santiago Quintero,3 José M. Cuyás,4 and Francisco Robaina5,7
1Department of Radiation Oncology-Research Unit, Las Palmas, Canary Islands, Spain
2Department of Medical Physics, Las Palmas, Canary Islands, Spain
3Department of Oral and Maxillofacial Surgery, Las Palmas, Canary Islands, Spain
4Department of Otolaryngology, Las Palmas, Canary Islands, Spain
5Department of Neurosurgery and Chronic Pain Unit of the Dr Negrín Hospital, Las Palmas, Canary Islands, Spain
6La Paterna Medical Center Las Palmas, Canary Islands, Spain
7Canary Islands Institute for Cancer Research (ICIC), Las Palmas, Canary Islands, Spain
Received 13 March 2004; Accepted 20 August 2004
Copyright © 2004 Bernardino Clavo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Advanced head and neck (H&N) tumors have a poor prognosis, and this is worsened by the occurrence of hypoxia and ischemia in the tumors. Ozonetherapy has proved useful in the treatment of ischemic syndromes, and several studies have described a potential increase of oxygenation in tissues and tumors. The aim of this prospective study was to evaluate the clinical effect of ozonetherapy in patients with advanced H&N cancer in the course of their scheduled radiotherapy. Over a period of 3 years, 19 patients with advanced H&N tumors who were undergoing treatment in our department with non-standard fractionated radiotherapy plus oral tegafur. A group of 12 patients was additionally treated with intravenous chemotherapy before and/or during radiotherapy. In the other group of seven patients, systemic ozonetherapy was administered twice weekly during radiotherapy. The ozonetherapy group was older (64 versus 54 years old, P = 0.006), with a higher percentage of lymph node involvement (71% versus 8%, P = 0.019) and with a trend to more unfavorable tumor stage (57% versus 8% IVb + IVc stages, P = 0.073). However, there was no significant difference in overall survival between the chemotherapy (median 6 months) and ozonetherapy (8 months) groups. Although these results have to be viewed with caution because of the limited number of patients, they suggest that ozonetherapy could have had some positive effect during the treatment of our patients with advanced H&N tumors. The adjuvant administration of ozonetherapy during the chemo–radiotherapy for these tumors merits further research.
Full article and citing articles available-
www.hindawi.com/journals/ecam/2004/581750/abs/ Evidence-Based Complementary and Alternative Medicine
Volume 1 (2004), Issue 1, Pages 93-98
dx.doi.org/10.1093/ecam/neh009 Original Article
Ozone Therapy for Tumor Oxygenation: a Pilot Study
Bernardino Clavo,1,5 Juan L. Pérez,2,5 Laura López,1,5 Gerardo Suárez,1,5 Marta Lloret,1,5 Victor Rodríguez,3 David Macías,2,5 Maite Santana,1,5 María A. Hernández,1 Roberto Martín-Oliva,2 and Francisco Robaina4,5
1Radiation Oncology and Research Unit, Las Palmas (Canary Islands), Spain
2Medical Physics, Las Palmas (Canary Islands), Spain
3La Paterna Medical Center, Las Palmas (Canary Islands), Spain
4Chronic Pain Unit, Dr Negrín Hospital, Las Palmas (Canary Islands), Spain
5Canary Islands Institute for Cancer Research (ICIC), Las Palmas (Canary Islands), Spain
Received 17 November 2003; Accepted 4 February 2004
Copyright © 2004 Bernardino Clavo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Tumor hypoxia is an adverse factor for chemotherapy and radiotherapy. Ozone therapy is a non-conventional form of medicine that has been used successfully in the treatment of ischemic disorders. This prospective study was designed to assess the effect of ozone therapy on tumor oxygenation. Eighteen subjects were recruited for the study. Systemic ozone therapy was administered by autohemotransfusion on three alternate days over one week. Tumor oxygenation levels were measured using polarographic needle probes before and after the first and the third ozone therapy session. Overall, no statistically significant change was observed in the tumor oxygenation in the 18 patients. However, a significant decrease was observed in hypoxic values ≤10 and ≤5 mmHg of pO2. When individually assessed, a significant and inverse non-linear correlation was observed between increase in oxygenation and the initial tumor pO2 values at each measuring time-point, thus indicating that the more poorly-oxygenated tumors benefited most (rho = −0.725; P = 0.001). Additionally, the effect of ozone therapy was found to be lower in patients with higher hemoglobin concentrations (rho = −0.531; P < 0.034). Despite being administered over a very short period, ozone therapy improved oxygenation in the most hypoxic tumors. Ozone therapy as adjuvant in chemo-radiotherapy warrants further research.
Full article and citing articles available-
www.hindawi.com/journals/ecam/2004/437019/citations/ Mediators Inflamm. 1998;7(5):313-7.
Studies on the biological effects of ozone: 8. Effects on the total antioxidant status and on interleukin-8 production.
Bocci V1, Valacchi G, Corradeschi F, Fanetti G.
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Abstract
Ozone (O3) is a controversial gas because, owing to its potent oxidant properties, it exerts damaging effects on the respiratory tract and yet it has been used for four decades as a therapy. While the disinfectant activity of O3 is understandable, it is less clear how other biological effects can be elicited in human blood with practically no toxicity. On the other hand plasma and cells are endowed with a powerful antioxidant system so that a fairly wide range of O3 concentrations between 40 and 80 microg/ml per gram of blood (approximately 0.83-1.66 mM) are effective but not deleterious. After blood ozonation total antioxidant status (TAS) and plasma protein thiol groups (PTG) decrease by 20% and 25%, respectively, while thiobarbituric acid reactive substances (TBARS) increases up to five-fold. The increase of haemolysis is negligible suggesting that the erythrocyte membrane is spared at the expense of other sacrificial substrates. While there is a clear relationship between the ozone dose and IL-8 levels, we have noticed that high TAS and PTG values inhibit the cytokine production. This is in line with the current idea that hydrogen peroxide, as a byproduct of O3 decomposition, acts as a messenger for the cytokine induction.
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J Biol Regul Homeost Agents. 1998 Jul-Sep;12(3):67-75.
Studies on the biological effects of ozone: 7. Generation of reactive oxygen species (ROS) after exposure of human blood to ozone.
Bocci V1, Valacchi G, Corradeschi F, Aldinucci C, Silvestri S, Paccagnini E, Gerli R.
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Abstract
The acceptance of any complementary medical approach is conditioned by the results obtained after the same scientific scrutiny applied in orthodox medicine. Otherwise any claim of efficacy remains in the realm of fiction. In the case of ozone therapy, the mechanisms of action have remained nebulous and in a series of publications we are trying to present the biochemical, immunological and morphological evidence in favour or against ozone therapy. We have now shown that ozone (O3) dissolved in the water of either plasma or serum or physiological saline generates reactive oxygen species (ROS), of which hydrogen peroxide (H2O2) can be unequivocally demonstrated by using specific methods for its detection. Lipids present in plasma preferentially those present in lipoproteins, undergo peroxidation that is somewhat O3-dose dependent and can be observed by the measurement of thiobarbituric acid reactive substances (TBARS). While the generation of H2O2 is crucial in activating both biochemical (hexose monophosphate shunt) and immunological (via the transcription factor NF-kB) mechanisms, the role of lipid oxidation products (LOP) remains to be investigated. We have shown here that there is a small but consistent induction of some cytokines (TNF-alpha, IFN-gamma and IL-2) when human blood is directly exposed to O3 concentrations up to 100 micrograms/ml per g of blood. On the other hand, isolated blood mononuclear cells (PBMC) in tissue culture medium are far more sensitive to the oxidant action of O3 as shown by a progressive reduction of the proliferation index with comparatively far lower O3, concentrations. On the whole, these results support the concept that much of the O3 toxicity is neutralized by the powerful antioxidant system of blood. The minimal hemolysis supports this idea but as far as platelets are concerned, we must mention that they tend to aggregate in heparinized blood, even when it is exposed to an O3 concentration of 40 micrograms/ml. In spite of the lack of side-effects after autohemotherapy, this drawback must be kept in mind and avoided in clinical practice.
Oxygen-ozone therapy in medicine: an update
www.ncbi.nlm.nih.gov/pubmed/19752...00m,isrctn
www.ncbi.nlm.nih.gov/pubmed/22470237Ozone therapy: A clinical review.
Elvis AM1, Ekta JS.
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Abstract
Ozone (O(3)) gas discovered in the mid-nineteenth century is a molecule consisting of three atoms of oxygen in a dynamically unstable structure due to the presence of mesomeric states. Although O(3) has dangerous effects, yet researchers believe it has many therapeutic effects. Ozone therapy has been utilized and heavily studied for more than a century. Its effects are proven, consistent, safe and with minimal and preventable side effects. Medical O(3) is used to disinfect and treat disease. Mechanism of actions is by inactivation of bacteria, viruses, fungi, yeast and protozoa, stimulation of oxygen metabolism, activation of the immune system. Medication forms in a gaseous state are somewhat unusual, and it is for this reason that special application techniques have had to be developed for the safe use of O(3). In local applications as in the treatment of external wounds, its application in the form of a transcutaneous O(3) gas bath has established itself as being the most practical and useful method, for example at low (sub-atmospheric) pressure in a closed system guaranteeing no escape of O(3) into the surrounding air. Ozonized water, whose use is particularly known in dental medicine, is optimally applied as a spray or compress. Diseases treated are infected wounds, circulatory disorders, geriatric conditions, macular degeneration, viral diseases, rheumatism/arthritis, cancer, SARS and AIDS.
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Does ozone therapy normalize the cellular redox balance? Implications for therapy of human immunodeficiency virus infection and several other diseases.
www.ncbi.nlm.nih.gov/pubmed/8692040 Mediators Inflamm. 2004 Dec;13(5-6):307-12.
Reversion by ozone treatment of acute nephrotoxicity induced by cisplatin in rats.
González R1, Borrego A, Zamora Z, Romay C, Hernández F, Menéndez S, Montero T, Rojas E.
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Abstract
BACKGROUND:
Ozone therapy has become a useful treatment for pathological processes, in which the damage mediated by reactive oxygen species is involved. Several lines of evidence suggest that cisplatin-induced acute nephrotoxicity is partially mediated by reactive oxygen species
AIMS:
To analyze the effect of ozone administration after cisplatin-induced acute nephrotoxicity.
METHODS:
Male Sprague-Dawley rats were treated with five intra-rectal applications of ozone/oxygen mixture at 0.36, 1.1 and 1.8 mg/kg after cisplatin intraperitoneal injection (6 mg/kg). Serum and kidneys were taken off 5 days after cisplatin treatment. Creatinine was measured in the serum and the activities of antioxidant enzymes and thiobarbituric acid reactive substances and glutathione content were analyzed in renal homogenate.
RESULTS:
Ozone treatment diminished the increase in serum creatinine levels, the glutathione depletion and also reversed the inhibition of superoxide dismutase, catalase and glutathione peroxidase activities induced by cisplatin in the rat kidney. Also, the renal content of thiobarbituric reactive substances was decreased by ozone/oxygen mixture applied after cisplatin.
CONCLUSION:
Intrarectal applications of ozone reversed the renal pro-oxidant unbalance induced by cisplatin treatment by the way of stimulation to some constituents of antioxidant system in the kidney, and thereby it decreased the renal damage.
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Mediators Inflamm. 2004 Feb;13(1):13-9.
Protection by ozone preconditioning is mediated by the antioxidant system in cisplatin-induced nephrotoxicity in rats.
Borrego A1, Zamora ZB, González R, Romay C, Menéndez S, Hernández F, Montero T, Rojas E.
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Abstract
BACKGROUND:
Acute renal failure is a dose-limiting factor of cisplatin chemotherapy. Here, we show the protective effect of ozone oxidative preconditioning against cisplatin-induced renal dysfunction in rats. Ozone oxidative preconditioning is a prophylactic approach, which favors the antioxidant-pro-oxidant balance for preservation of the cell redox state by increasing antioxidant endogenous systems in various in vivo and in vitro experimental models.
AIMS:
To analyze the protective role of ozone oxidative preconditioning against cisplatin-induced nephrotoxicity.
METHODS:
Male Sprague-Dawley rats were pretreated with 15 intrarectal applications of ozone/oxygen mixture at 0.36, 0.72, 1.1, 1.8 and 2.5 mg/kg before cisplatin intraperitoneal injection (6 mg/kg). Serum and kidneys were extracted and analyzed 5 days after cisplatin treatment for determinations of the renal content of glutathione, thiobarbituric acid-reactive substances, renal concentration and enzymatic activities of catalase, superoxide dismutase and glutathione peroxidase.
RESULTS:
Ozone pretreatment prevented the increase in serum creatinine levels, the glutathione depletion and the inhibition of superoxide dismutase, catalase and glutathione peroxidase activities induced by cisplatin in the rat kidney. Also, the renal content of thiobarbituric acid-reactive substances was decreased by ozone therapy. These protective effects of ozone were dose dependent.
CONCLUSIONS:
Intrarectal ozone therapy prevented effectively the renal antioxidant unbalance induced by cisplatin treatment.
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Sultan Qaboos Univ Med J. 2014 Aug;14(3):e342-8. Epub 2014 Jul 24.
Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats.
Delgado-Roche L1, Hernández-Matos Y1, Medina EA1, Morejón DÁ1, González MR2, Martínez-Sánchez G3.
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Abstract
OBJECTIVES:
Induced dilated cardiomyopathy is the main limitation of the anti-cancer drug doxorubicin, which causes oxidative stress and cardiomyocyte death. As ozone therapy can activate the antioxidant systems, this study aimed to investigate the therapeutic efficacy of ozone-oxidative preconditioning against doxorubicin-induced cardiotoxicity.
METHODS:
The study was carried out from September 2013 to January 2014. Sprague-Dawley rats were randomly distributed in the following treatment groups: Group 1 were treated with 2 mg/kg intraperitoneal (i.p.) of doxorubicin twice a week for 50 days; Group 2 were treated with 0.3 mg of ozone/oxygen mixture at 50 μg/mL of ozone per 6 mL of oxygen by rectal insufflation and then treated with doxorubicin; Group 3 were treated as Group 2 but only with the oxygen, and Group 4 were treated with oxygen first, and then with sodium chloride i.p. as the control group.
RESULTS:
The results showed that ozone therapy preserved left ventricle morphology which was accompanied by a reduction of serum pro-brain natriuretic peptide levels. The cardioprotective effects of ozone-oxidative preconditioning were associated with a significant increase (P <0.05) of antioxidant enzymes activities and a reduction of lipid and protein oxidation (P <0.05).
CONCLUSION:
Ozone-oxidative preconditioning prevents doxorubicin-induced dilated cardiomyopathy through an increase of antioxidant enzymes and a reduction of oxidised macromolecules. This establishes the background for future studies to determine if ozone therapy can be used as a complementary treatment for attenuating doxorubicin-induced cardiotoxicity in cancer patients.
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I still have numerous other medical studies on the benefits of ozone therapy.
Also look in to betulinic acid, another powerful antiviral and anticancer agent, derived from the chaga mushroom, with lot of medical studies backing it as well for cancer.
I also really like dried and aged chaparral, which is antiviral, immune supporting, the strongest natural antioxidant known and a great mitosis inhibitor. So, it is great for cancer. I normally combine it with the antiviral and anticancer herbs pau d' arco (lapacho) and andrographis.
Turkey tail mushrooms contain the cancer fighting compounds polysaccharides K and P as well as organic germanium.
Suma supports immunity and is the highest herbal source of anticancer organic germanium.
Rooibos tea (honey bush, red bush, red tea) is a great antioxidant, being 30 times stronger than green tea, and without the high tannin and fluoride of green tea. Rooibos contains a superoxide dismutase like compound that is very effective for cancer, inflammation and boosting immunity.
Amla is a great source of vitamin C to support immunity and to decrease inflammation. Amla also increases anti-cancer superoxide dismutase levels by around 80%.