Post by 1dave on Mar 20, 2019 10:39:13 GMT -5
www.internationalskeptics.com/forums/showthread.php?t=6561
sorgoth Muse
Join Date: Aug 2002 Posts: 977
Can DNA be extracted from Oil?
I was just thinking about how oil is organic matter, deep in the earth, decomposed, ect. when I wondered: Would there be a way to tell what life the oil came from? What species, specifically?
Join Date: Aug 2002 Posts: 977
Can DNA be extracted from Oil?
I was just thinking about how oil is organic matter, deep in the earth, decomposed, ect. when I wondered: Would there be a way to tell what life the oil came from? What species, specifically?
Jon_in_london Illuminator
Join Date: Aug 2002 Posts: 4,989
Well, not really from the oil but.........
www.ncbi.nlm.nih.gov/entrez/q...&holding=f1000
Quote:
Microorganisms inhabiting subterranean oil fields have recently attracted much attention. Since intact groundwater can easily be obtained from the bottom of underground oil-storage cavities without contamination by surface water, studies on such oil-storage cavities are expected to provide valuable information to understand microbial ecology of subterranean oil fields. RESULTS: DNA was extracted from the groundwater obtained from an oil-storage cavity situated at Kuji in Iwate, Japan, and 16S rRNA gene (16S rDNA) fragments were amplified by PCR using combinations of universal and Bacteria-specific primers. The sequence analysis of 154 clones produced 31 different bacterial sequence types (a unique clone or group of clones with sequence similarity of > 98). etc etc
Mind you, there are bugs that live on crude oil so the answer may be yes but not in the way you want.
Join Date: Aug 2002 Posts: 4,989
Well, not really from the oil but.........
www.ncbi.nlm.nih.gov/entrez/q...&holding=f1000
Quote:
Microorganisms inhabiting subterranean oil fields have recently attracted much attention. Since intact groundwater can easily be obtained from the bottom of underground oil-storage cavities without contamination by surface water, studies on such oil-storage cavities are expected to provide valuable information to understand microbial ecology of subterranean oil fields. RESULTS: DNA was extracted from the groundwater obtained from an oil-storage cavity situated at Kuji in Iwate, Japan, and 16S rRNA gene (16S rDNA) fragments were amplified by PCR using combinations of universal and Bacteria-specific primers. The sequence analysis of 154 clones produced 31 different bacterial sequence types (a unique clone or group of clones with sequence similarity of > 98). etc etc
Mind you, there are bugs that live on crude oil so the answer may be yes but not in the way you want.
biology.stackexchange.com/questions/31831/does-crude-oil-or-kerogen-contain-ancient-dna
Does crude oil or kerogen contain ancient DNA?
Ask Question - 6
According to wikipedia by the formation of oil there are a lot of organic materials present including DNA. Is it possible that this DNA is conserved somehow for millions of years?
(I think the DNA part has been edited out)
1 Answer
According to Live Science, the temperatures are in the 120 to 300 degree-Fahrenheit range (48-148 degree-Celcius) when crude oil is forming. DNA in "dry" conditions completely degrades at temperatures above 190 degree-Celcius. Which means that there could be DNA in the oil itself if there are some surviving cells, however, outside the cells there are DNases which will chew up DNA.
So the DNA they are talking about is like the DNA studied by Gong et. al, where they're looking at the live bacteria in the crude oil, which would be descendants of the bacteria that was around when the oil first began its degradation from organic material.
On both NCBI and PLOS ONE there appear to be a number of papers studying the bacterial growth in crude oil. Which would be why you may have seen articles about DNA in the oil.
